Biotin-tyramide

- Overview
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    Background
    
    Biotin-tyramide is used for tyramide signal amplification (TSA) which is a powerful, patented technology that significantly enhances both chromogenic and fluorescent signals. TSA is easily integrated into standard nonradioactive in situ hybridization (ISH) or IHC protocols.
    TSA is an enzyme-mediated detection method that uses horseradish peroxidase (HRP) to catalyze the deposition of a fluorophore-labeled tyramide amplification reagent onto tissue sections or cell preparation surfaces that have been previously blocked with proteins.
    Biotin-tyramide use horseradish peroxidase (HRP) to catalyze covalent deposition of biotin labels directly adjacent to the immobilized enzyme. The labeling reaction is quick (less than 10 minutes) and deposited labels can be detected with streptavidin conjugates for imaging in brightfield or fluorescence microscopy.
- Properties
  - Categories
    
    Reagent used for tyramide signal amplification (TSA)
    
    Alternative Name
    
    Biotin-Phenol,Biotin Phenol; N-(4-hydroxyphenethyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide
    
    CAS Number
    
    41994-02-9
    
    Molecular Formula
    
    C18H25N3O3S
    
    Molecular Weight
    
    363.47
    
    Appearance
    
    A solid
    
    Purity
    
    98.00%
    
    Solubility
    
    insoluble in H2O; ≥100.2 mg/mL in DMSO; ≥8.18 mg/mL in EtOH with ultrasonic
    
    Storage
    
    Store at -20°C
    
    SMILES
    
    O=C1N[[email protected]@]([H])([[email protected]]([H])(CS2)N1)[[email protected]@H]2CCCCC(NCCC3=CC=C(O)C=C3)=O
    
    * For Research Use Only
- Reference
  - 1. Wei Qin, Samuel A. Myers, et al. "Spatiotemporally-resolved mapping of RNA binding proteins via functional proximity labeling reveals a mitochondrial mRNA anchor promoting stress recovery." Nat Commun. 2021 Aug 17;12(1):4980. PMID:34404792
    2. Clemens AM, Lenschow C, et al. "Estrus-Cycle Regulation of Cortical Inhibition." Curr Biol. 2019 Feb 18;29(4):605-615.e6. PMID:30744972
    3. Liu P, Huang J, et al. "Mammalian mitochondrial RNAs are degraded in the mitochondrial intermembrane space by RNASET2." Protein Cell. 2017 Jul 20. PMID:28730546