Biotin-dPEG®₇-azide

Biotin-dPEG®7-azide, product number 10825, is part of a family of biotinylation products designed for azide-alkyne click chemistry reactions. These reactions require catalytic amounts of copper(I) or ruthenium or the use of highly strained cyclooctyne compounds such as dibenzylcyclooctyne (DBCO). This family of products enables the biotinylation of molecules using chemistry that is different from traditional amide bond formation. Consequently, these products permit new types of biotinylated constructs.

What are dPEG® Products?
Quanta BioDesign invented dPEG® technology. Our single molecular weight polyethylene glycol products have no dispersity (Ð = 1). In contrast, conventional PEG products typically have relatively large dispersity values (Ð = 1.05 – 1.2).
Biotinylation
Biotinylation is the process of covalently attaching biotin to a molecule such as a peptide, protein, or nucleic acid, or a surface such as glass or gold. The process is used widely in bioconjugation research and product development. Although biotin is poorly soluble in water, the conjugation of biotin to an amphiphilic dPEG® linker imparts excellent solubility in aqueous and organic solvents.

Using Biotin-dPEG®7-azide
By design, Biotin-dPEG®7-azide works in all azide-alkyne cycloadditions. These reactions are catalyzed by copper(I) (CuAAC) or ruthenium (RuAAC) or promoted by strain (SPAAC) using DBCO or like reagents.

Through protein engineering, non-natural amino acids with alkyne side chains can be incorporated into specific locations on proteins, thereby enabling reproducible biotinylation of proteins. Likewise, synthetic peptides containing amino acids with alkyne side chains allow azide-alkyne click chemistry-driven biotinylation. Furthermore, surfaces functionalized with alkyne groups can be biotinylated using click chemistry. Moreover, this product can be modified with other products from Quanta BioDesign to create new compounds tailored to your specific needs.

As a biotinylation reagent, Biotin-dPEG®11-azide works in various applications. Pull-down assays, affinity purification, plate-type assays such as ELISA, and supramolecular construction can all incorporate Biotin-dPEG®7-azide.

Because Biotin-dPEG®7-azide is a single molecular weight dPEG® product, analysis of the resulting conjugates is simplified. The researcher using this product does not have to analyze a variety of PEG chain lengths and molecular weights. Instead, the well-defined dPEG® product will yield predictable, identifiable conjugates. When using Biotin-dPEG®7-azide with proteins or peptides engineered to contain alkyne side chains at specific locations, the location of each conjugation sites is predictable.
Biotin-dPEG®7-azide and Related Products
Biotin-dPEG®7-azide is just one member of a family of Biotin-dPEG®-azide products. Also, we offer several DBCO-functionalized dPEG® products as SPAAC partners for our Click dPEG® Biotinylation Reagents.

Please contact us at for specific academic pricing.