Biotin-dPEG®₁₁-azide

Biotin-dPEG®11-azide, product number 10784, is part of a family of biotinylation products designed for use in azide-alkyne click chemistry reactions. This product enables biotinylation of molecules using either copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC), ruthenium-catalyzed azide-alkyne cycloaddition (RuAAC), or strain-promoted azide-alkyne cycloaddition (SPAAC) chemistry. Consequently, these products allow new types of biotinylated constructs.

Discrete PEG Products
Quanta BioDesign invented dPEG® technology. Our single molecular weight polyethylene glycol products have no dispersity (Ð = 1). In contrast, conventional PEG products typically have relatively large dispersity values (Ð = 1.05 – 1.2).
Biotinylation
Biotinylation is the process of covalently attaching biotin to a molecule such as a peptide, protein, or nucleic acid, or a surface such as glass or gold. The process is used widely in bioconjugation research and product development. Although biotin is poorly soluble in water, the conjugation of biotin to an amphiphilic dPEG® linker imparts excellent solubility in aqueous and organic solvents.

Using Biotin-dPEG®11-azide
Biotin-dPEG®11-azide is designed for click chemistry reactions. Catalytic amounts of copper(I) or ruthenium or highly-strained cyclooctyne reagents such as dibenzylcyclooctyne (DBCO) promote a rapid reaction between the azide and an alkyne group to form a triazole moiety.

Through protein engineering, non-natural amino acids with alkyne side chains can be incorporated into specific locations on proteins, thereby enabling reproducible biotinylation of proteins. Likewise, synthetic peptides containing amino acids with alkyne side chains allow azide-alkyne click chemistry-driven biotinylation. Furthermore, surfaces functionalized with alkyne groups can be biotinylated using click chemistry. Moreover, this product can be modified with other products from Quanta BioDesign to create new compounds tailored to your specific needs.

As a biotinylation reagent, Biotin-dPEG®11-azide works in various applications. Pull-down assays, affinity purification, plate-type assays such as ELISA, and supramolecular construction can all incorporate Biotin-dPEG®11-azide. For instance, in a novel use of PN10784, Biotin-dPEG®11-azide was employed in a pull-down assay to identify electrophiles in signaling pathways in the worm Caenorhabditis elegans.

Because Biotin-dPEG®11-azide is a single molecular weight dPEG® product, analysis of the resulting conjugates is simplified. The researcher using this product does not have to analyze a variety of PEG chain lengths and molecular weights. Instead, the well-defined dPEG® product will yield predictable, identifiable conjugates. When using Biotin-dPEG®11-azide with proteins or peptides engineered to contain alkyne side chains at specific locations, both the number and location of conjugation sites are predictable.

Products Related to Biotin-dPEG®11-azide
Biotin-dPEG®11-azide is just one member of a family of Biotin-dPEG®-azide products. Also, we offer several DBCO-functionalized dPEG® products as SPAAC partners for our Click dPEG® Biotinylation Reagents.

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