Biotin-dPEG®₂₃-azide

Biotin-dPEG®23-azide, product number 10787, is part of a family of biotinylation products used in azide-alkyne click chemistry reactions. This family of products enables biotinylation of molecules using chemistry other than the traditional amide bond formation. Consequently, these products allow new types of biotinylated constructs.

Biotinylation
Biotinylation is the process of covalently attaching biotin to a molecule such as a peptide, protein, or nucleic acid, or a surface such as glass or gold. It is widely used in bioconjugation research and product development.

Biotin usually is poorly soluble in water, but the amphiphilic dPEG® linker imparts excellent solubility in water or aqueous buffer and organic solvents. The azide reactive group at the other end of the molecule reacts with an alkyne in a reaction that can be catalyzed by copper(I), ruthenium, or strain (for example, using a product containing dibenzylcyclooctyne, known by the acronym DBCO).

Biotin-dPEG®23-azide has numerous uses. Through protein engineering, non-natural amino acids with alkyne side chains can be incorporated into distinct locations on proteins, thereby enabling site-specific, reproducible biotinylation of proteins. Likewise, synthetic peptides can be constructed that allow azide-alkyne click chemistry-driven biotinylation of the peptides. Also, surfaces functionalized with alkyne groups can be biotinylated using click chemistry. Furthermore, this product can be modified with other products from Quanta BioDesign to create new compounds tailored to the customer's specific needs. For example, in a 2018 study published in the journal Langmuir, researchers from The BioDesign Institute at Arizona State University used Biotin-dPEG®23-azide to construct a Y-shaped, bivalent, bis-biotin structure with a rigid core to probe the interaction of biotin and streptavidin using both atomic force microscopy (AFM) and surface plasmon resonance (SPR).

As a biotinylation reagent, Biotin-dPEG®23-azide functions like other biotinylation reagents. For example, pull-down assays, affinity purification, ELISA and other plate-type assays, and supramolecular construction can employ Biotin-dPEG®23-azide successfully. Many other applications using Biotin-dPEG®23-azide are possible.

Biotin-dPEG®23-azide simplifies the analysis of conjugates made with it because it is a single molecular weight dPEG® product. The researcher using this product does not have to analyze a variety of PEG chain lengths and molecular weights. Instead, the well-defined dPEG® product yields predictable, identifiable conjugates. Moreover, if this product reacts with proteins or peptides engineered to contain alkyne side chains at specific locations, both the number and location of the conjugation sites are predictable.

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