Biotin Coated - 96 well plates - 8 Well Strips on 12 x 8 frame

Biotin Plates 96-Well Strip Plates: Designed with 12 strips of 8 wells on a rigid frame, preventing misorientation for consistent and accurate assays.

Biotin coated surfaces offer a powerful instrument to carry out one of the most useful interactions in immunochemistry that involves the specificity binding of biotin to avidin, streptavidin or neutravidin. This binding shows a great constant affinity (10-15 M).

Biotin Coated 96 Well Plates are pre-blocked in order to minimize any non-specific binding and to ensure long-term stability.

Biotin Plates are available in clear, white, and black polystyrene in Breakable Strip, Strip and Solid format. They are suitable for ELISA, Luminescence, Fluorescence, and Chemiluminescent assays.

Key features of Biotin Plates
· Biotin-binding applications: It facilitates highly specific interactions with avidin, streptavidin, neutravidin, or other biotin-binding proteinsBiotin surface: the bind shows a great constant affinity (10–15 M) and is highly stable interaction.
· Blocking: Plates are pre blocked with standard proteic blocking (ELISA Blocking) or with non proteic blocking (BlockerWell)
· Low Fluorescence Polystyrene: Manufactured with pure high-quality polystyrene, minimizing background noise and improving optical clarity.
· Wells design: flat bottom.
· Multiple Formats: Available in breakable strip plates, non-breakable strip plates, and solid plates for flexibility in applications.
· Colors: Available in clear, white, and black. Customizable upper rim colors upon request.
Reduced Crosstalk: White and black plates minimize well-to-well light scatter, improving signal accuracy in luminescence and fluorescence assays.
Uniform Optical Properties: Clear plates are ideal for spectrophotometric readings and imaging, providing consistent and accurate light transmission.
· Automation-Compatible: Easily integrated with high-throughput automated systems for liquid handling, washing, and analysis.
· Alphanumeric Coding: For easy and accurate well identification during experiments.
· Recommended working volume: of 75 to 200 μl
· Packaging: Each coated plate is packed in a single barrier bag with desiccant
· Unit: Contains 5 plates
· Minimum order: 10 plates.
· Ready to use

Key Benefits
· Plates Design:
The mould design provides optical quality, important to reduce the background signal.
The rim protects the external face of the bottom from scratches.
· Improved Washing Efficiency: Radius-edged well bottoms enhance washing, reducing residual materials and improving assay consistency.
· Reduced Cross-Contamination: Designed to minimize the risk of cross-contamination between wells, ensuring precise sample isolation.
· Enhanced Imaging: The flat surface provides a clear focal plane, essential for high-quality imaging in microscopy, especially for fluorescence and phase-contrast analysis.
· Improved Mixing: The flat-bottom design facilitates better sample mixing, ensuring consistent results across assays.

Please contact us at for specific academic pricing.

Background

Biotin Coated Surface offers a powerful instrument to carry out one of the most useful interactions in immunochemistry that involves the specificity binding of biotin to avidin, streptavidin or neutravidin.
Biotin coated surfaces offer a powerful instrument to carry out one of the most useful interactions in immunochemistry that involves the specificity binding of biotin to avidin, streptavidin or neutravidin. This binding shows a great constant affinity (10-15 M).

Biotin, or vitamin H (M.W. 244.31 Da), is a small naturally occurring cofactor that is present in every living cell in very minute amounts (usually less than 0.0001 %). The biotin molecule normally exists bound to proteins (such as pyruvate carboxylase) through its valeric acid carboxylic group by an amide bond to lysine side-chain amines.

The polystyrene optical features don't change, allowing the modified surface to be used as a valid tool to carry out biological tests.

· Examples of applications for Biotin Coated surface
interaction with avidin
interaction with streptavidin
interaction with neutravidin

· Biotin Coated Surfaces main features
Biomat Biotin Coated products have the following characteristics:
Ready to Use
Manufactured under ISO:9001 guidelines
All lots are tested for uniformity and reproducibility
Certificate of Quality is released for every lot
For Research Use Only
Also, Biotin Coated Products ensures:

Uniformity
The Biotin Coated Products show a CV% less than 5 when streptavidin-HRP is used as detector and TMB as substrate in an ELISA format.

Storage and Stability
The Biotin Coated products, if unopened, are stable at 2 – 8°C until the expiration date printed on the label. If opened, store in a closed pouch with desiccant and use within the expiration date.

· Biotin Coated Microplates Specifications
Coating
Biotin (M.W. 244.31 Da) is coated using 200 μl/well.

Binding specificity
The binding specificity of Biotin Coated 96-Well Plate was evaluated towards Streptavidin-HRP conjugate. Click on the following link to see the results.