Biotin Coated - 96 well Solid plates

Biotin Plates 96-Well Solid Plates: Solid format designed for high-performance assays.

Biotin coated surfaces offer a powerful instrument to carry out one of the most useful interactions in immunochemistry that involves the specificity binding of biotin to avidin, streptavidin or neutravidin. This binding shows a great constant affinity (10-15 M).

Biotin Coated 96 Well Plates are pre-blocked in order to minimize any non-specific binding and to ensure long-term stability.

Biotin Plates are available in clear, white, and black polystyrene in Breakable Strip, Strip and Solid format. They are suitable for ELISA, Luminescence, Fluorescence, and Chemiluminescent assays.

Surface:
The Biotin surface provides a robust platform for applications involving biotin-binding proteins, including avidin, streptavidin, and neutravidin enhancing the precision and reliability of binding assays. This bind shows a great constant affinity (10-15 M) and is highly stable interaction.

Key features of Biotin Plates
· Biotin-binding applications: It facilitates highly specific interactions with avidin, streptavidin, neutravidin, or other biotin-binding proteinsBiotin surface: the bind shows a great constant affinity (10–15 M) and is highly stable interaction.
· Blocking: Plates are pre blocked with standard proteic blocking (ELISA Blocking) or with non proteic blocking (BlockerWell)
· Low Fluorescence Polystyrene: Manufactured with pure high-quality polystyrene, minimizing background noise and improving optical clarity.
· Wells design: flat bottom.
· Multiple Formats: Available in breakable strip plates, non-breakable strip plates, and solid plates for flexibility in applications.
· Colors: Available in clear, white, and black. Customizable upper rim colors upon request.
Reduced Crosstalk: White and black plates minimize well-to-well light scatter, improving signal accuracy in luminescence and fluorescence assays.
Uniform Optical Properties: Clear plates are ideal for spectrophotometric readings and imaging, providing consistent and accurate light transmission.
· Automation-Compatible: Easily integrated with high-throughput automated systems for liquid handling, washing, and analysis.
· Alphanumeric Coding: For easy and accurate well identification during experiments.
· Recommended working volume: of 75 to 200 μl
· Packaging: Each coated plate is packed in a single barrier bag with desiccant
· Unit: Contains 5 plates
· Minimum order: 10 plates.
· Ready to use

Key Benefits
· Plates Design:
The mould design provides optical quality, important to reduce the background signal.
The rim protects the external face of the bottom from scratches.
· Improved Washing Efficiency: Radius-edged well bottoms enhance washing, reducing residual materials and improving assay consistency.
· Reduced Cross-Contamination: Designed to minimize the risk of cross-contamination between wells, ensuring precise sample isolation.
· Enhanced Imaging: The flat surface provides a clear focal plane, essential for high-quality imaging in microscopy, especially for fluorescence and phase-contrast analysis.
· Improved Mixing: The flat-bottom design facilitates better sample mixing, ensuring consistent results across assays.

Please contact us at for specific academic pricing.

Background

Biotin Coated Surface offers a powerful instrument to carry out one of the most useful interactions in immunochemistry that involves the specificity binding of biotin to avidin, streptavidin or neutravidin.
Biotin coated surfaces offer a powerful instrument to carry out one of the most useful interactions in immunochemistry that involves the specificity binding of biotin to avidin, streptavidin or neutravidin. This binding shows a great constant affinity (10-15 M).

Biotin, or vitamin H (M.W. 244.31 Da), is a small naturally occurring cofactor that is present in every living cell in very minute amounts (usually less than 0.0001 %). The biotin molecule normally exists bound to proteins (such as pyruvate carboxylase) through its valeric acid carboxylic group by an amide bond to lysine side-chain amines.

The polystyrene optical features don't change, allowing the modified surface to be used as a valid tool to carry out biological tests.

· Examples of applications for Biotin Coated surface
interaction with avidin
interaction with streptavidin
interaction with neutravidin

· Biotin Coated Surfaces main features
Biomat Biotin Coated products have the following characteristics:
Ready to Use
Manufactured under ISO:9001 guidelines
All lots are tested for uniformity and reproducibility
Certificate of Quality is released for every lot
For Research Use Only
Also, Biotin Coated Products ensures:

Uniformity
The Biotin Coated Products show a CV% less than 5 when streptavidin-HRP is used as detector and TMB as substrate in an ELISA format.

Storage and Stability
The Biotin Coated products, if unopened, are stable at 2 – 8°C until the expiration date printed on the label. If opened, store in a closed pouch with desiccant and use within the expiration date.

· Biotin Coated Microplates Specifications
Coating
Biotin (M.W. 244.31 Da) is coated using 200 μl/well.

Binding specificity
The binding specificity of Biotin Coated 96-Well Plate was evaluated towards Streptavidin-HRP conjugate. Click on the following link to see the results.