Streptavidin Coated - 96 well plates - 8 Well Strips on 12 x 8 frame

Streptavidin Plates 96-Well Strip Plates: Designed with 12 strips of 8 wells on a rigid frame, preventing misorientation for consistent and accurate assays.

The Streptavidin Plates are ideal for binding a wide range of biotinylated molecules, making them highly recommended for ELISA and other immunoassays. Streptavidin is one of the most popular biotin-binding proteins in research, with each of its four subunits capable of binding a biotin molecule, resulting in enhanced assay sensitivity and reliability. The streptavidin-coated surface provides a versatile and powerful platform for capturing biotinylated antibodies, antigens, proteins, peptides, polysaccharides, oligonucleotides, DNA fragments, and more. This coating is especially useful for molecules that may not adhere reliably through passive adsorption or that require specific orientation for optimal binding. Using Streptavidin Coated Plates for ELISA ensures improved assay performance, with higher sensitivity and reduced background noise, leading to more accurate and consistent results.

Streptavidin Coated Plates are pre-blocked in order to minimize any non-specific binding and to ensure long-term stability.

Streptavidin Coated 96 Well Plates are available in clear, white, and black polystyrene in Breakable Strip, Strip and Solid format. They are suitable for ELISA, Luminescence, Fluorescence, and Chemiluminescent assays.

Surface:
The Streptavidin Coated Surface on Streptavidin Coated 96 Well Solid Plates provides a highly effective and versatile platform for binding biotinylated molecules, including proteins, peptides, polysaccharides, oligonucleotides, and DNA fragments. Streptavidin's strong affinity for biotin allows for robust and stable interactions, making it particularly valuable for applications where passive adsorption is unreliable or where molecules may adsorb in an unfavorable orientation. These plates ensure consistent and oriented binding, which is critical for enhancing assay sensitivity and accuracy in ELISA, nucleic acid capture, and other biochemical assays. Additionally, the high specificity of streptavidin-biotin binding minimizes background noise, offering improved signal clarity in complex assay systems.

Key features of Streptavidin High Binding Plates
· High Binding Streptavidin vs. Streptavidin coated surface: particularly suited for measurement of biotinylated low molecular weight molecules.
· Blocking: Plates are pre blocked with standard proteic blocking (ELISA Blocking) or with non proteic blocking (BlockerWell)
· Low Fluorescence Polystyrene: Manufactured with pure high-quality polystyrene, minimizing background noise and improving optical clarity.
· Wells design: flat bottom.
· Multiple Formats: Available in breakable strip plates, non-breakable strip plates, and solid plates for flexibility in applications.
· Colors: Available in clear, white, and black. Customizable upper rim colors upon request.
Reduced Crosstalk: White and black plates minimize well-to-well light scatter, improving signal accuracy in luminescence and fluorescence assays.
Uniform Optical Properties: Clear plates are ideal for spectrophotometric readings and imaging, providing consistent and accurate light transmission.
· Automation-Compatible: Easily integrated with high-throughput automated systems for liquid handling, washing, and analysis.
· Alphanumeric Coding: For easy and accurate well identification during experiments.
· Recommended working volume: of 50 to 100 μl
· Packaging: Each coated plate is packed in a single barrier bag with desiccant
· Unit: Contains 5 plates
· Minimum order: 10 plates.
· Ready to use

Key Benefits
· Plates Design:
The mould design provides optical quality, important to reduce the background signal.
The rim protects the external face of the bottom from scratches.
· Improved Washing Efficiency: Radius-edged well bottoms enhance washing, reducing residual materials and improving assay consistency.
· Reduced Cross-Contamination: Designed to minimize the risk of cross-contamination between wells, ensuring precise sample isolation.
· Enhanced Imaging: The flat surface provides a clear focal plane, essential for high-quality imaging in microscopy, especially for fluorescence and phase-contrast analysis.
· Improved Mixing: The flat-bottom design facilitates better sample mixing, ensuring consistent results across assays.

Please contact us at for specific academic pricing.

Background

Streptavidin Coated Surface is a powerful and universal instrument for binding any biotinylated molecule.
Streptavidin is the most popular and widely available biotin-binding protein for research methods. It is a tetrameric protein (M.W. 60 kDa) with very high affinity for biotin (Ka=10-15 M). Biotin is a small molecule which can be conjugated to many proteins without losing or altering their activity. Each protein can bind many biotin molecules. The bond is the strongest known non-covalent biological interaction.

The Streptavidin Coating is a surface treatment that allows to bind any biotinylated molecule (Antibodies – Antigens – Proteins – Peptides – Polysaccharides – Oligonucleotides – DNA fragments etc.). It finds a special application for those molecules which do not offer reliable bonding by passive adsorption or adsorb in an unfavorable orientation. For example, Streptavidin Coated Plates are suitable with ELISA assays protocol.

Each subunit of Streptavidin binds one molecule of Biotin giving at Streptavidin Coated Surface a greater assay sensitivity. Streptavidin-Biotin binding main features are specificity, stability, and affinity.

· Streptavidin Coated Surfaces main features
Biomat's Streptavidin Coated plates and tubes have the following characteristics:
Ready to use
Manufactured under ISO:9001 guidelines
Post-coated (blocked) for low non specific binding and long-term stability
All lots are tested for uniformity and reproducibility
Certificate of Quality is released for every lot
For Research Use Only

Also, Streptavidin Coated Products ensures:

Uniformity
The Streptavidin Coated products show a CV% less than 5 using biotin-HRP as detector and TMB as substrate.

Storage and Stability
The Streptavidin coated products, if unopened, are stable at 2-8°C until the expiration date printed on the label. If opened, store in closed pouch with desiccant and use within the expiration date.

· Streptavidin Coated Microplates Specifications
Coating
Streptavidin is coated using 200 µl/well.

· Streptavidin Coated Plates parameters and functional features
The following parameters were analysed to determine the functional features of the Streptavidin coated plates,

Binding capacity towards Biotin.
Specificity towards Biotin.
Binding capacity towards Biotinylated IgG.
Stability tests:
Endurance under strong chemical contacts.
Shelf life at 37°C.
Temperature stress (transport simulation).
Long storage.
Binding capacity towards Biotin.

1. Binding capacity towards Biotin
Streptavidin coated wells (and BSA saturated control wells) were incubated with a calibrated Biotin solution. Subsequently, aliquots of this solution, concomitantly with Biotin standards, were mixed with biotinylated peroxidase and transferred into new empty Streptavidin coated wells. From the amount of enzyme bound to the solid phase, the Biotin content of the samples was calculated.

This value was compared with the amount of biotin originally added; from the difference (corrected for-non specific binding of biotin to the control wells), the capacity of the wells for biotin was derived.

2. Specificity towards biotin
Streptavidin Coated Wells were incubated with solutions of biotinylated peroxidase and non biotinylated peroxidase (blanks) as a catcher in a ELISA format using TMB as substrate.

3. Binding capacity towards biotinylated HIgG
Streptavidin coated wells were incubated with biotinylated HIgG in an ELISA format using HIgG-HRP as a detector and TMB as substrate.

4. Stability tests
Endurance under strong chemical contacts