Overview

Bestarose 4FF(Fast Flow)and Bestarose 6FF are SEC resins made via emulsification, cleaning and sieving on 4% and 6% agarose, respectively. They are used for separation, purification, or detection and biological macromolecules including polysaccharides, nucleic acids and viruses. This resin is often used as a matrix for affinity, HIC and IEX resins. Bestarose 4FF and Bestarose 6FF are made on Bestarose 4B and Bestarose 6B base matrix via bifocal crosslinkers. In addition, its degree of crosslinking is higher than that of Bestarose CL-4B and Bestarose CL-6B, enabling better physical and chemical properties, 121°C autoclaving and fast flow velocity, which is suitable for large-scale separation and purification applications.

Product		Bestarose 4FF	Bestarose 6FF
Appearance		White slurry
Agarose concentration		4%	6%
Separation range	Linear numerator	30KD~5000KD	10KD~1000KD
	Globular molecule	60KD~20000KD	10KD~4000KD
Particle size +		45~165μm
Average particle size		~90μm
Pressure flow velocity⁺⁺		200-300cm/h (0.1MPa BXK50/60,H=25cm,20°C,in water)	≥200cm/h (0.1MPa BXK50/60,H=25cm,20°C,in water)
Max. pressure		0.3MPa (3bar)
pH stability		2~12 (working),2~14 (CIP)
Chemical stability		Stable in common aqueous buffers: 0.5M NaOH+++,2M NaOH+++,6M GuHCl,8M Urea, 1M acetic acid, 30% acetonitrile	Stable in common aqueous buffers: 0.5M NaOH+++,2M NaOH+++,1M acetic acid, 30% acetonitrile, 8M Urea, 6M GuHCl
Sterilization		Can tolerate 121°C autoclaving (20min)
Storage++++		2~30°C, 20% ethanol or 2% benzyl alcohol

+Particle size is normally distributed, and particles within this range account for more than 95% of the total.
++ The pressure flow velocity describes the relationship between pressure and flow under the set circumstances. The pressure given shall not be taken as the maximum pressure of the resin.
+++0.5M NaOH and 2M NaOH only be used for cleaning.
++++ 2% benzyl alcohol is only used for international transport or special requirements from customer.

Please contact us at for specific academic pricing.