Overview

Bestarose 4B and Bestarose 6B are SEC resin made through emulsification, washing and sieving on the basis of 4% and 6% agarose. They are used for the separation, purification and detection of biological macromolecules including polysaccharides, nucleic acids and viruses. This resin is often used as a matrix for affinity, HIC and IEX resins. The gel structure of Bestarose 4B and Bestarose 6B is fixed by hydrogen bonding, contributing to relatively soft matrix, poor pressure resistance, and poor resistant to autoclaving.

Product		Bestarose 4B	Bestarose 6B
Appearance		White slurry
Agarose concentration		4%	6%
Separation range	Linear numerator	30KD~5000KD	10KD~1000KD
	Globular molecule	60KD~20000KD	10KD~4000KD
Particle size+		45~165μm
Average particle size		~90μm
Pressure flow velocity++		70~140cm/h (Δp/L=15 BXK50/20, H=10cm,20°C,in water)	≥100cm/h (Δp/L=15 BXK50/20, H=10cm,20°C,in water)
Max. pressure		0.16bar	0.2bar
pH stability		4~9 (working),4~9 (CIP)
Chemical stability		Stable in common aqueous buffers: 4M urea, 70% ethanol
Sterilization+++		0.5M NaOH ,2h
Operating temperature		Operating temperature: 2~40°C, do not freeze
Storage++++		2~30°C,20% ethanol or 2% benzyl alcohol
Avoid		Freezing may damage the internal structure of the resin

+Particle size is normally distributed, and particles within this range account for more than 95% of the total.
++The pressure/flow characteristics describes the relationship between pressure and flow under the set circumstances. The pressure given shall not be taken as the maximum pressure of the resin. +++This operation does not guarantee product stability.
++++2% benzyl alcohol is only used for international transport or special requirements from customer.

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