BcMag™ Secreted His-tagged protein purification Kit

BcMag™ Secreted His-tagged protein purification kit is based on magnetic beads coupled with a unique, proprietary ligand loaded with nickel ions. The ligands are extraordinarily firmly bonded and have a high affinity for His-tagged proteins, and exhibit low metal, ion-leaching properties even in the presence of chemical additives such as chelators (EDTA), strong reducing agents (DTT), or components of cell culture supernatants, which typically strip off Ni ions and reduce the functionality of most IMAC magnetic beads. The His-tagged protein purification resins allow the efficient purification of recombinant polyhistidine-tagged proteins directly from a soluble intracellular protein extract, HeLa, CHO mammalian cells, or Sf9 insect cells culture supernatant. They can be used manually with a magnetic stand or automatically with an instrument. It avoids extensive and time-consuming sample pretreatment processes, such as buffer exchange by dialysis in conjunction with concentration operations. Magnetic resins have significant advantages over traditional chromatography such as columns, agarose, or non-magnetic resin. The magnetic bead-based format enables rapid high-yield processing of 96 samples in about 20 minutes, achieving more than 85% purities. When using column-based technologies, processing multiple samples in academic research labs may necessitate a significant quantity of hand pipetting. This pipetting can discourage differences in the yield of target biomolecules between experiments and people. Staff and students may require extensive training and practice to produce constant protein yields. It is due to the numerous benefits of magnetic resins, such as their ease of use, rapid experimental protocols, suitability, and convenience for high throughput automated and miniaturized processing.

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