BcMag™ Protein G Terbium Fluorescent Magnetic Beads

Super Bright Protein G - Terbium Fluorescent Magnetic Beads offer a solution to the limitations of conventional fluorophores. These beads are perfect for ultra-sensitive Time-Resolved FRET Assay, providing higher fluorescence intensity, lower background signals, and reduced issues such as self-quenching, sensitivity to environmental factors, fluorescence intermittency, and hydrophobicity-related aggregation.

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Background

BcMag™ TR-FRET (Time-Resolved FRET) Assay
BcMag™ TR-FRET Assay, in contrast to typical FRET (Förster Resonance Energy Transfer) assays, uses time-resolved Fluorescence magnetic beads (BcMag™ TR-Magnetic Beads) as the donor fluorophore. The donor and acceptor can be two proteins, two DNA strands, an antigen, an antibody, or a ligand and its receptor. After a reasonable time delay (usually 50 to 100 s), a signal is generated by fluorescence resonance energy transfer between a donor and an acceptor molecule when they are close and monitored in a time-resolved way. In BcMag™ TR-FRET Assay, a trace amount of analytes can be easily enriched from the complex by TR-Magnetic Beads, resulting in higher sensitivity. This assay practically eliminates all fluorescence backgrounds caused by the sample and plastic microplate, as well as by direct acceptor excitation. As a result, the signal-to-noise ratios of the BcMag™ TR-FRET Assay are very high, and the background is quite low. Furthermore, the assay does not need washing steps. BcMag™ TR-FRET Assay offers substantial advantages to bioassays in high throughput screening, such as assay flexibility, dependability, increased assay sensitivity, higher throughput, and fewer false positive/false negative results.
Terbium cryptate fluorophore is an efficient Fluorescence label due to its distinct specific properties. It is excited at 320nm and emits green fluorescence at 545nm, with a long fluorescence lifetime (1050 μsec) and large stokes shifts (220 nm). By taking advantage of these properties, time-resolved fluorescence measurement can dramatically reduce the fluorescence background from the sample and increase the signal-to-noise ratio to offer detectability better than one order of magnitude than conventional Fluorescence dyes. BcMag™ Protein G Terbium Fluorescence Magnetic Beads are excellent donors used in TR-FRET assays.