BcMag™ Protein L Europium Fluorescent Magnetic Beads

Discover how Super Bright Protein L Europium Fluorescent Magnetic Beads can enhance your Time-Resolved FRET Assays by providing higher fluorescence intensity, lower background signals, and overcoming limitations such as self-quenching, environmental sensitivity, fluorescence intermittency, and hydrophobicity-related aggregation.

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Background

BcMag™ TR-FRET (Time-Resolved FRET) Assay
BcMag™ TR-FRET Assay, in contrast to typical FRET (Förster Resonance Energy Transfer) assays, uses time-resolved Fluorescence magnetic beads (BcMag™ TR-Magnetic Beads) as the donor fluorophore. The donor and acceptor can be two proteins, two DNA strands, an antigen, an antibody, or a ligand and its receptor. After a reasonable time delay (usually 50 to 100 s), a signal is generated by fluorescence resonance energy transfer between a donor and an acceptor molecule when they are close and monitored in a time-resolved way. In BcMag™ TR-FRET Assay, a trace amount of analytes can be easily enriched from the complex by TR-Magnetic Beads, resulting in higher sensitivity. This assay practically eliminates all fluorescence backgrounds caused by the sample and plastic microplate, as well as by direct acceptor excitation. As a result, the signal-to-noise ratios of the BcMag™ TR-FRET Assay are very high, and the background is quite low. Furthermore, the assay does not need washing steps. BcMag™ TR-FRET Assay offers substantial advantages to bioassays in high throughput screening, such as assay flexibility, dependability, increased assay sensitivity, higher throughput, and fewer false positive/false negative results.
Europium cryptate fluorophore is an efficient time-resolved Fluorescence tag due to its distinct specific properties. It is excited at 340nm and emits red fluorescence at 615nm, with a long fluorescence lifetime (730 μsec) and large stokes shifts (275 nm). By taking advantage of these properties, time-resolved fluorescence measurement can dramatically reduce the fluorescence background from the sample and increase the signal-to-noise ratio to offer detectability better than one order of magnitude than conventional Fluorescence dyes. BcMag™ Protein L Europium Fluorescence Magnetic Beads are excellent donors used in TR-FRET assays.