BcMag™ One-Step PCR Inhibitor Removal Kit

For this reason, Bioclone developed a novel, highly efficient PCR inhibitor removal system based on magnetic beads – BcMag™ One-Step PCR Inhibitor Removal Kit provides one-step removal of PCR inhibitor from impure DNA samples before PCR, RT, and other downstream applications based on negative chromatography. The magnetic beads are superparamagnetic and modified with our proprietary chemistry. When mixed with inhibitor-containing samples, the beads instantly capture and remove the PCR inhibitors. At the same time, only the pure DNA remains in the solution and is ready for all downstream applications. The beads can effectively remove many common inhibitors such as polyphenolic compounds, humic/fulvic acids, acidic polysaccharides, tannins, melanin, heparin, detergents, and denim dyes, and divalent cations such as Ca2+, Mg2+, etc.

Workflow
The protocol is straightforward and fast: one tube, one step, and one minute. Add the magnetic beads directly to the pre-purified DNA samples and vortex or pipette to capture and remove the impurities. After vortexing/pipetting, the beads are magnetically removed, while the supernatant contains the purified and ready-to-run products. Unlike standard bind-wash-elute protocol, this convenient procedure does not contain traces of organic solvents, chaotropic salts, or EDTA and is almost 100% DNA recovery. The beads enable 96 samples to be processed simultaneously in less than 10 minutes with cost-effective lab vortex mixers.

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