BcMag™ Pure Midiprep Plasmid DNA Purification Kit

Plasmids are circular, extrachromosomal DNA molecules that act as carriers, or vectors, for a specific DNA segment in molecular biology. Bacteria are employed to reproduce plasmids, allowing your desired DNA to be mass-produced. The method by which researchers acquire plasmids from bacteria is known as plasmid purification. Purifying plasmid DNA from bacterial cells is a critical step in cloning. Bacterial cells are lysed during plasmid purification, which liberates DNA and other biological components from the cell wall. After removing cellular components, the DNA-containing lysate is treated to eliminate impurities and separate the plasmid DNA from the genomic DNA. The plasmid DNA extracted is the same quality as that produced by purification with two passes through cesium chloride gradients—the most stringent approach for DNA purification. High-quality DNA can be purified in less than twenty minutes for transfections and other applications. There is no need for extra processes to remove impurities such as RNA, proteins, or endotoxins. Furthermore, the protocol’s exclusion of phenol, chloroform, ethidium bromide, and cesium chloride reduces exposure to and disposal of hazardous compounds.

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