-
-
Overview
-
Isolation of DNA from blood and other body fluids in adequate quantities is integral to the clinic and forensic research and analysis. Large-scale clinical point-of-care tests require a quick and efficient DNA isolation technique for a trace amount of blood sample or other body fluids. However, current blood DNA purification protocols suffer drawbacks, including low yield, compromised quality, cost, tedious and time-consuming, toxic organic solvents, and many more. To overcome these drawbacks, we developed a novel revolutionary one-step DNA purification system based on magnetic beads and negative chromatography, which combines DNA extraction with removing all the PCR inhibitors from the samples without performing DNA isolation and purification steps. BcMag™ One-Step Blood DNA Purification Kit allows rapid and efficient purification of genomic DNA from whole blood, serum, plasma, or other body fluids. The kit uses our unique proprietary magnetic beads to efficiently lyse cells and remove all impurities simultaneously in an aqueous buffer, leaving the DNA untouched. The procedure employs mild lysis conditions, avoiding harsh conditions such as alkaline lysis and toxic chemicals for lysing cells to maintain DNA integrity and the time-consuming cleanup of organic solvent from the sample. Furthermore, the magnetic beads eliminate PCR inhibitors from samples in a single step without DNA extraction. It increases DNA integrity, boosts nucleic acid yields, and minimizes DNA loss caused by typical DNA purification techniques’ time-consuming “bind-wash-elute” procedure. Following sample lysis, the straightforward one-step purification technique enables simultaneous processing of 96 samples and produces pure DNA in less than 30 minutes. Purified genomic DNA has the highest integrity and can be used in various downstream applications such as qPCR, STR, etc.
Please contact us at for specific academic pricing.
-
- Properties
- Applications
-
Overview
PCR inhibitor removal by magnetic beads
BcMag™ One-Step Blood DNA Purification Kit
BcMag™ One-Step Blood DNA Purification Kit
Catalog Number:
NAPK567591BIO
Mfr. No.:
AF101; AF102
Price:
$544
Size:
Quantity:
Add to Cart: