BcMag™ Cleavable Tosyl-Activated Magnetic Beads

BcMag™ Cleavable Tosyl-Activated Magnetic Beads are uniform magnetic beads grafted with tosyl functional groups on the surface.). The tosyl-activated magnetic beads can efficiently conjugate ligands containing primary amine in either aqueous or organic solvents (30% DMF) without introducing any charge. Since the active tosyl group is linked with the beads through a built-in cleavable disulfide linker, after affinity purification, reducing agents such as DTT or β-mercaptoethanol can cleave and separate the target molecule-ligand complex from the beads. The cleavable tosyl magnetic beads are ideal matrices for conjugating large-size proteins or small peptides. The Tosyl-Activated magnetic resins are an ideal choice for covalently attaching antibodies, peptides, complete proteins, and functional enzymes to the surface. The immobilized beads are widely used in the Immunoprecipitation of proteins and protein complexes due to their low background and covalent binding of antibodies to the bead surface. The Tosyl Activated magnetic resins coupling reaction is carried out at 37°C, and pH ranges from neutral to high. We advocate coupling at pH 8.5-9.5, but coupling with pH labile ligands can be done in an alternate buffer at pH 7.4. The unique dry form eliminates the need for acetone solvent storage or removal and disposal. Furthermore, because the dry resin concentrates the sample as it swells, lowering the volume of the starting material and resulting in highly effective ligand immobilization, it is perfect for coupling reactions with dilute materials.

Workflow
The Beads perfectly as affinity resin for affinity purification to refine molecules, cells, and parts of cells into purified fractions. After conjugation with ligands, add the beads to a sample containing the target molecules, then mix, incubate, wash and elute the target molecules.

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