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APPBP1/6xHis-UBA3

APPBP1/6xHis-UBA3

Catalog Number:
EUAE097621UBP
Mfr. No.:
B1400
Price:
$300
  • Size:
    25 µg
    Quantity:
    Add to Cart:
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      • Overview

        • APPBP1/UBA3 is the heterodimeric E1 enzyme that conjugates Nedd8 to protein substrates. Nedd8 is conjugated onto the catalytic cysteine residue of UBA3 by formation of a thioester bond in an ATP-dependent reaction. The activated Nedd8 is then passed on to an E2 (Ubc12) to be finally attached to a substrate. Nedd8 is mainly conjugated on Cullins to increase the activity of the Cullin – RING E3 Ub ligases.

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      • Properties
        • Protein Name
          APPBP1 (NAE1;HPP1; ula-1; A-116A10.1); UBA3 (UBE1C; hUBA3; MGC22384; DKFZp566J164)
          Source
          Bacterial recombinant
          Formulation
          20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
          Storage
          Store at -80°C; avoid multiple freeze-thaw cycles
          Concentration
          See tube label
          Molecular Weight
          APPBP1 (60.2 kDa), UBA (51.8 kDa)
          Sequence
          NCBI RefSeq: NM_003905 (APPBP1); NM_003968 (UBA3)

          * Research Use Only

    Datasheet
    MSDS

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