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Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified 6xHis-SAE1/SAE2

Coomassie-stained SDS-PAGE Lane 1: Molecular weight markers Lane 2: 5 µg purified 6xHis-SAE1/SAE2

6xHis-SAE1/SAE2

6xHis-SAE1/SAE2

Catalog Number:

EUAE097622UBP

Mfr. No.:

B1301

Price:

$584

Size:

100 µg

Quantity:

Add to Cart:

- Overview
  - SAE1/SAE2 is the heterodimeric E1 enzyme that conjugates SUMO to protein substrates. The conjugation process is very similar to that of protein ubiquitination. SUMO is conjugated onto the catalytic cysteine residue of SAE2 by formation of a thioester bond in an ATP-dependent reaction. The activated SUMO is then passed on to an E2 (Ubc9) to be finally attached to a substrate.
    
    Please contact us at for specific academic pricing.
- Properties
  - Protein Name
    
    SAE1 (AOS1; SUA1; UBLE1A; HSPC140; FLJ3091); SAE2 (RBBP8; RIM; CTIP; JWDS; SCKL2)
    
    Source
    
    Bacterial recombinant
    
    Formulation
    
    20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
    
    Storage
    
    Store at -80°C; avoid multiple freeze-thaw cycles
    
    Concentration
    
    See tube label
    
    Molecular Weight
    
    SAE1 (33 kDa), SAE2 (71.2 kDa)
    
    Sequence
    
    NCBI RefSeq: NM_005500 (SAE1); NM_002894 (SAE2)
    
    * Research Use Only

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