Antibody Methotrexate Conjugation Kit

This kit for antibody (IgG) labeling of methotrexate (formerly known as amethopterin) for early stage screening of your antibody.

Methotrexate (MTX) is a chemotherapeutic agent and immune system suppressant on the World Health Organization's List of Essential Medicines (the most effective and safe medicines needed in a health system). This kit includes methotrexate N-hydroxysuccimide ester, which can be directly coupled to the antibody in a single step. The product is purified to remove any unreacted drug.

This kit provides materials to conjugate 1-3 mg of one (CM11407x1) or three (CM11407x3) antibody samples (IgG) with methotrexate.

If you have a smaller amount of IgG, non-IgG type antibody, or other customization needs, please select the customization radio button and/or fill out the information above.

• Drug Information
Name: Methotrexate (amethopterin)
CAS number: 59-05-2
Mechanism of action: Competitively inhibits dihydrofolate reductase (DHFR), an enzyme that participates in tetrahydrofolate synthesis. Folic acid is essential for de novo synthesis of the nucleoside thymidine. In addition, folate is essential for biosynthesis of purine and pyrimidine bases. By inhibiting DHFR, methotrexate inhibits the synthesis of DNA, RNA, thymidylates, and proteins.
Medical usage: Breast cancer, leukemia, lung cancer, lymphoma, and osteosarcoma

• Key Features of this PerKit™
Offers a simple and easy way to label IgG with methotrexate with minimum exposure to the chemotherapeutic drug
Stable linkage
Single conjugation step, fast and easy preparation: 6 h preparation with <1 h hands-on time
All reagents and supplies included for preparation and purification
DAR with average 3 to 5 methotrexate per antibody
Included stabilizing buffer for long-term storage
>99% conjugated products by size-exclusion chromatography (SEC) and free of any unreacted drugs

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