Antibody Doxorubicin Conjugation Kit

This kit for antibody (IgG) labeling of doxorubicin (Dox) for early stage screening of your antibody.

Doxorubicin is an anticancer drug on the World Health Organization's List of Essential Medicines (the most effective and safe medicines needed in a health system). This kit is designed to label any antibody (IgG type) with doxorubicin via succinic acid linker (N-succinyl doxorubicin acid is included in the kit). The user supplies the antibody. Using the kit components, the user converts the carboxylic acid of doxorubicin to an activated sulfo N-hydroxysuccinimide ester (NHS ester), followed by reaction with the surface amines of the antibody. The product is then purified to remove any unreacted drug.

This kit provides materials to conjugate 1-3 mg of one (CM11406x1) or three (CM11406x3) antibody samples (IgG) with doxorubicin (Dox).

If you have a smaller amount of IgG, non-IgG type antibody, or other customization needs, please select the customization radio button and/or fill out the information above.

• Drug Information
Name: Doxorubicin (trade name: Adriamycin)
CAS number: 23214-92-8
Mechanism of action: Intercalation of DNA and inhibition of macromolecular biosynthesis.
Medical usage: Breast cancer, bladder cancer, Kaposi's sarcoma, lymphoma, and acute lymphocytic leukemia.
ADC usage: Doxorubicin has been linked to an antibody and found to be effective in treating lung cancer (Ref. Trail, PA et al. 1993, Science, 261, 212-215)

• Requirement for Antibody
1. Preferably > 90% pure by gel electrophoresis
2. Total amount: 1-3 mg (protein content as measured by UV).

• Key Features of this PerKit™
Offers a simple and easy way to label IgG with Doxorubicin with minimum expose to the chemotherapeutic drug
Stable linkage
Fast and easy preparation: 4 h preparation and <1 h hands-on time
DAR with average 4 Dox per antibody
Included stabilization buffer for long-term storage
More than 99% conjugated products (free of any unreacted drugs)

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