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Overview
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This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. A pseudogene, related to this gene, is also located on chromosome 18.
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- Properties
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Overview
Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug ROCK1 antibody (STJ29833). Western blot was performed from the immunoprecipitate using ROCK1 antibody (STJ29833) at a dilition of 1:1000.
Western blot analysis of extracts from normal (control) and ROCK1 knockout (KO) HeLa cells, using ROCK1 antibody (STJ29833) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 3s.
Immunohistochemistry of paraffin-embedded mouse spleen using ROCK1 antibody (STJ29833) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using ROCK1 antibody (STJ29833) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.
Anti-ROCK1 antibody
Anti-ROCK1 antibody
Catalog Number:
A001037745STJ
Mfr. No.:
STJ29833
Price:
$295
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