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Overview
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The protein encoded by this gene contains a RING finger, a motif known to be involved in protein-protein and protein-DNA interactions. This protein was reported to interact with the tumor suppressor protein RB1. Studies of the rat counterpart suggested that this protein may function as an E3 ubiquitin-protein ligase, and facilitate the ubiquitination and degradation of syntaxin 1, which is an essential component of the neurotransmitter release machinery. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.
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- Properties
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Overview
Immunofluorescence analysis of U2OS cells using RNF40 antibody (STJ28526).
Chromatin immunoprecipitation analysis of extracts of HeLa cells, using RNF40 antibody (STJ28526) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Western blot analysis of extracts of various cell lines, using RNF40 antibody (STJ28526) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.
Anti-RNF40 antibody
Anti-RNF40 antibody
Catalog Number:
A001036620STJ
Mfr. No.:
STJ28526
Price:
$297
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