STJ71044 (1µg/ml) staining of HeLa cell lysate (35µg protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Detected by chemiluminescence.

Anti-PRPF31 antibody

Catalog Number:

A001042839STJ

Mfr. No.:

STJ71044

Price:

$499

Size:

100 µg

Quantity:

Add to Cart:

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- Overview
  - Please contact us at for specific academic pricing.
- Properties
  - Host
    
    goat
    
    Antibody Type
    
    polyclonal
    
    Reactivity
    
    human; mouse; rat; dog; pig; cow
    
    Purification
    
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    
    Conjugate
    
    Unconjugated
    
    Formulation
    
    Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
    
    Storage
    
    Store at -20°C and avoid freeze-thaw cycles.
    
    Concentration
    
    0.5 mg/ml
    
    Regulatory Status
    
    RUO
    
    Antigen
    
    U4/U6 Small Nuclear Ribonucleoprotein Prp31; Pre-Mrna-Processing Factor 31; Serologically Defined Breast Cancer Antigen Ny-Br-99; U4/U6 Snrnp 61 Kda Protein; Protein 61k; Hprp31 (Gene ID:26121; UniProt:Q8WWY3)
    
    * For research use only
- Applications
  - Application
    
    ELISA; WB
    
    Application Description
    
    ELISA-antibody detection limit dilution 1:128000.
    WB-Approx 60kDa band observed in lysates of cell lines A431 and HeLa. Recommended concentration: 1-3µg/ml. Primary incubation was 1 hour. An additional band of unknown identity was also consistently observed at 35kDa. This band was successfully blocked by incubation with the immunising peptide. Also a band of 28kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal.