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Overview
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Plastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. In humans, two ubiquitous plastin isoforms (L and T) have been identified. Plastin 1 (otherwise known as Fimbrin) is a third distinct plastin isoform which is specifically expressed at high levels in the small intestine. The L isoform is expressed only in hemopoietic cell lineages, while the T isoform has been found in all other normal cells of solid tissues that have replicative potential (fibroblasts, endothelial cells, epithelial cells, melanocytes, etc. ). The C-terminal 570 amino acids of the T-plastin and L-plastin proteins are 83% identical. It contains a potential calcium-binding site near the N terminus. Alternate splicing results in multiple transcript variants.
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Overview
Immunohistochemistry of paraffin-embedded human stomach using PLS3 antibody (STJ25032) at dilution of 1:100 (40x lens).
Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug PLS3 antibody (STJ25032). Western blot was performed from the immunoprecipitate using PLS3 antibody (STJ25032) at a dilition of 1:1000.
Western blot analysis of extracts of various cell lines, using PLS3 antibody (STJ25032) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.
Anti-PLS3 antibody
Anti-PLS3 antibody
Catalog Number:
A001034704STJ
Mfr. No.:
STJ25032
Price:
$297
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