Name: Anti-PLA2G4A antibody
Price: 499.00 USD

- Overview
  - Please contact us at for specific academic pricing.
- Properties
  - Host
    
    goat
    
    Antibody Type
    
    polyclonal
    
    Reactivity
    
    human; mouse; rat
    
    Purification
    
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    
    Conjugate
    
    Unconjugated
    
    Formulation
    
    Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
    
    Storage
    
    Store at -20°C and avoid freeze-thaw cycles.
    
    Concentration
    
    0.5 mg/ml
    
    Regulatory Status
    
    RUO
    
    Antigen
    
    Cytosolic Phospholipase A2; Cpla2; Phospholipase A2 Group Iva Includes-Phospholipase A2; Phosphatidylcholine 2-Acylhydrolase-Lysophospholipase (Gene ID:5321; UniProt:P47712)
    
    * For research use only
- Applications
  - Application
    
    ELISA; WB; IF; Flow Cytometry; IHC
    
    Application Description
    
    ELISA-antibody detection limit dilution 1:32000.
    WB-Approx 80-85kDa band observed in lysates of cell line A549. Preliminary experiments also showed an 85-90kDa band in lysates of cell lines A431, HeLa and NIH-3T3 Recommended concentration:0.3-1µg/ml. Primary incubation 1 hour at room temperature.Preliminary testing was unsuccessful on Rat for this particular batch.
    IHC-Paraffin embedded Human Uterus. Recommended concentration: 3.75µg/ml.
    IF-Strong expression of the protein seen in the cytoplasm of A549 cells. Recommended concentration: 10µg/ml.
    FC-Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml.