STJ70673 (4µg/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

Anti-PIWI/HIWI antibody

Catalog Number:

A001042478STJ

Mfr. No.:

STJ70673

Price:

$499

Size:

100 µg

Quantity:

Add to Cart:

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- Overview
  - Please contact us at for specific academic pricing.
- Properties
  - Host
    
    goat
    
    Antibody Type
    
    polyclonal
    
    Reactivity
    
    human; mouse; rat; dog; pig
    
    Purification
    
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    
    Conjugate
    
    Unconjugated
    
    Formulation
    
    Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
    
    Storage
    
    Store at -20°C and avoid freeze-thaw cycles.
    
    Concentration
    
    0.5 mg/ml
    
    Regulatory Status
    
    RUO
    
    Antigen
    
    Piwi-Like Protein 1 (Gene ID:9271; UniProt:Q96J94)
    
    * For research use only
- Applications
  - Application
    
    ELISA; IHC
    
    Application Description
    
    ELISA-antibody detection limit dilution 1:64000.
    WB-Preliminary experiments showed a 100kDa band but also bands at approx 35kDa and 26-28kDa in Human Ovary, Placenta, Prostate and Testis lysates after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the additional bands we observe given the calculated size of 98.6kDa according to NP_004755.2. All detected bands were successfully blocked by incubation with the immunizing peptide.
    IHC-In paraffin embedded Human Testis shows textured cytoplasm staining in all germ cells. Recommended concentration: 4-6µg/ml.