-
-
Overview
-
PCNA is a protein encoded by the PCNA gene which is approximately 28, 7 kDa. PCNA is localised to the nucleus. It is involved in the telomere C-strand synthesis and trans-lesion synthesis. It is found in the nucleus and is a cofactor of DNA polymerase-delta. It acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. PCNA is expressed in the liver, lung, bone marrow, muscle and kidney. Mutations in the PCNA gene may result in ataxia-telangiectasia-like disorder. STJ96933 was developed from clone 12D10 and was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. This antibody detects endogenous PCNA protein.
Please contact us at for specific academic pricing.
-
- Properties
- Applications
-
Overview
IHC staining of Human breast cancer tissue paraffin-embedded, diluted at 1:200 Immunofluorescence analysis of Rat-testis tissue. 1, PCNA monoclonal antibody (12D10) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B Western blot analysis of Hela (1), Rat brain (2), NIH 3T3 (3), 293T (4), diluted at 1:5000. cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).
Immunofluorescence analysis of Human-lung-cancer tissue. 1, PCNA monoclonal antibody (12D10) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1, PCNA monoclonal antibody (12D10) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-testis tissue. 1, PCNA monoclonal antibody (12D10) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1, PCNA monoclonal antibody (12D10) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunofluorescence analysis of Hela cell. 1, 14-3-3 Theta/Tau (phospho Ser232) Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). (red) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).
Anti-PCNA antibody
Anti-PCNA antibody
Catalog Number:
A001052302STJ
Mfr. No.:
STJ96933
Price:
$224
Size:
Quantity:
Add to Cart:
.