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Overview
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Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20.
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Overview
Immunohistochemistry of paraffin-embedded human liver cancer using NCL Rabbit pAb (STJ29927) at dilution of 1:100 (40x lens).
Confocal immunofluorescence analysis of U2OS cells using NCL Polyclonal Antibody (STJ29927) at dilution of 1:100. Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using NCL antibody (STJ29927) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.
Anti-NCL antibody
Anti-NCL antibody
Catalog Number:
A001037811STJ
Mfr. No.:
STJ29927
Price:
$297
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