Name: Anti-Mouse IL2RA In Vivo Antibody - Low Endotoxin (PC61)
Price: 680.00 USD

- Overview
  - Antigen Distribution: The CD25 antigen is detected on CD4 positive T-cells but not on CD8 positive T-cells. However, when stimulated with phytohemagglutinin (PHA), the CD25 antigen can be detected on CD8 positive T cells. The CD25 antigen is also expressed on CD20 positive B cells. The CD25 antigen is negative on CD 56 positive natural killer cells, CD 14 positive monocytes, granulocytes, red blood cells and platlets. The CD25 antigen is expressed on malignant cells of adult T-cell leukemias (ATL's) carring HTLV-1, most cases of cutaneous T cell lymphoma and lymphoepithelioid lymphomas among non-Hodgkin's T cell lymphomas.
    Specificity: Anti-CD25 In Vivo Antibody - Low Endotoxin (PC61) recognizes an epitope on Mouse IL-2 RA
    Purification method: This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
    Isotype Control: Rat IgG1 Isotype Control for In Vivo - Low Endotoxin [GL113] (ICH2246)
    Endotoxin: ≤ 1.0 EU/mg as determined by the LAL method
    Aggregation: Aggregation level ≤ 5%
    
    Please contact us at for specific academic pricing.
    
    Background
    
    IL-2 receptor alpha chain (IL-2Ralpha) is a component of high-affinity IL-2 receptors and thus is a key regulator of lymphocyte proliferation. IL-2R alpha expression occurs at specific stages of early T and B lymphocyte development and is induced upon activation of mature lymphocytes. IL-2R alpha is essential for regulation of both the size and content of the peripheral lymphoid compartment, probably by influencing the balance between clonal expansion and cell death following lymphocyte activation. IL-2Ralpha is also important in the formation of high-affinity binding sites, and the inducibility of the alpha gene contributes to the highly regulated and transient display of high-affinity IL-2R.
- Properties
  - Host
    
    rat
    
    Isotype
    
    IgG1 lambda
    
    Reactivity
    
    mouse
    
    Clone
    
    PC61
    
    Immunogen
    
    IL-2-dependent cytolytic mouse T-cell clone B6.1
    
    Purification
    
    >95% by SDS-PAGE and HPLC
    
    Formulation
    
    0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4 with no carrier protein, potassium or preservatives added.
    
    Storage
    
    anti-CD25 In Vivo Antibody - Low Endotoxin (PC61) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
    
    Molecular Weight
    
    ≥ 2.0 mg/ml
    
    Regulatory Status
    
    Each investigator should determine their own optimal working dilution for specific applications.
    
    Antigen
    
    (Gene ID:Interleukin-2 receptor subunit alpha, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, Il2ra, p55, CD25)
    
    * Products are for research use only. Not for use in diagnostic or therapeutic procedures.
- Applications
  - Application
    
    Immunoprecipitation; WB; Functional Assays; Blocking; Flow Cytometry; IHC (frozen)

Anti-Mouse IL2RA In Vivo Antibody - Low Endotoxin (PC61)

Anti-Mouse IL2RA In Vivo Antibody - Low Endotoxin (PC61)

Background

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