Name: Anti-Mouse CD32/CD16 In Vivo Antibody - Low Endotoxin (2.4G2)
Price: 680.00 USD

- Overview
  - Antigen Distribution: The Fc gamma II/III receptors are present on mouse natural killer cells, monocytes, macrophages, granulocytes, B-cells and most fetal thymocytes.
    Specificity: Anti-CD32/CD16 In Vivo Antibody - Low Endotoxin (2.4G2) recognizes the FcγII and FcγIII receptors
    Purification method: This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
    Isotype Control: Rat IgG2b In Vivo Isotype Control - Low Endotoxin [1-2] (ICH2243)
    Endotoxin: ≤ 1.0 EU/mg as determined by the LAL method
    Aggregation: Aggregation level ≤ 5%
    
    Please contact us at for specific academic pricing.
    
    Background
    
    CD16 is a low-affinity IgG Fc receptor III and CD32 is FcR II. CD16/CD32 are expressed on B cells, monocytes/macrophages, NK cells, granulocytes, mast cells, and dendritic cells. The Fc receptors bind antibody-antigen immune complexes and mediate adaptive immune responses. Clone 2.4G2 antibody is used in flow cytometry staining experiments to prevent non-specific binding of IgG to the FcγIII and FcγII, and possibly FcγI, receptors prior to staining with antigen-specific primary antibodies. The Fab fragments of the 2.4G2 antibody have also been used to block Fc receptors in vivo.
- Properties
  - Host
    
    rat
    
    Isotype
    
    IgG2b
    
    Reactivity
    
    mouse
    
    Clone
    
    2.4G2
    
    Purification
    
    >95% by SDS-PAGE and HPLC
    
    Formulation
    
    0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
    
    Storage
    
    anti-CD32/CD16 In Vivo Antibody - Low Endotoxin (2.4G2) is stable for at least four (4) weeks when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
    
    Molecular Weight
    
    0.5 mg/ml
    
    Regulatory Status
    
    Each investigator should determine their own optimal working dilution for specific applications.
    
    Antigen
    
    (Gene ID:Low affinity immunoglobulin gamma Fc region receptor II, Fcgr2, Fc-gamma-RIIB, FcRII, IgG Fc receptor II beta, Ly-17, Low affinity immunoglobulin gamma Fc region receptor III, Fcgr3, FcRIII, Fc-gamma RIII)
    
    * Products are for research use only. Not for use in diagnostic or therapeutic procedures.
- Applications
  - Application
    
    WB; Blocking; Flow cytometry; IHC (frozen); Immunoprecipitation; CODEX

Anti-Mouse CD32/CD16 In Vivo Antibody - Low Endotoxin (2.4G2)

Anti-Mouse CD32/CD16 In Vivo Antibody - Low Endotoxin (2.4G2)

Background

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