Anti-Mouse CD45 In Vivo Antibody - Low Endotoxin (I3/2.3)

Anti-Mouse CD45 In Vivo Antibody - Low Endotoxin (I3/2.3)

Catalog Number:
A001437238ICH
Mfr. No.:
ICH1057
Price:
$680
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      • Overview
        • Antigen Distribution: The CD45 antigen is present on all cells of hematopoietic origin, but not erythrocytes
          Specificity: Anti-CD45 In Vivo Antibody - Low Endotoxin (I3/2.3) recognizes an epitope on Mouse CD45
          Purification method: This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
          Isotype Control: Rat IgG2b In Vivo Isotype Control - Low Endotoxin [1-2] (ICH2243)
          Endotoxin: ≤ 1.0 EU/mg as determined by the LAL method
          Aggregation: Aggregation level ≤ 5%

          Please contact us at for specific academic pricing.

          Background

          CD45 is a 180-240 kD glycoprotein also known as the leukocyte common antigen (LCA). CD45 plays a key role in TCR and BCR signal transduction.

      • Properties
        • Host
          rat
          Isotype
          IgG2b
          Reactivity
          mouse
          Clone
          I3/2.3
          Immunogen
          Mouse lymphoma cell line
          Purification
          >95% by SDS-PAGE and HPLC
          Formulation
          0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
          Storage
          anti-CD45 In Vivo Antibody - Low Endotoxin (I3/2.3) is stable when stored at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
          Molecular Weight
          1.0 - 5.0 mg/ml
          Regulatory Status
          Flow Cytometry: For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume.
          Each investigator should determine their own optimal working dilution for specific applications.

          * Products are for research use only. Not for use in diagnostic or therapeutic procedures.

      • Applications
        • Application
          WB; Flow Cytometry; IHC (frozen); IHC (paraffin)

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