Anti-Mouse CD3e In Vivo Antibody - Low Endotoxin (500A2)

Anti-Mouse CD3e In Vivo Antibody - Low Endotoxin (500A2)

Catalog Number:
A001437223ICH
Mfr. No.:
ICH1040
Price:
$680
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      • Overview
        • Antigen Distribution: The CD3 complex is present on thymocytes and mature T-lymphocytes of all mouse strains tested.
          Specificity: Anti-CD3e In Vivo Antibody - Low Endotoxin (500A2) recognizes an epitope on Mouse CD3ε
          Purification method: This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
          Endotoxin: <1.0 EU/mg as determined by the LAL method
          Aggregation: Aggregation level ≤ 5%

          Please contact us at for specific academic pricing.

          Background

          CD3 subunits gamma, delta and epsilon are required for proper assembly, trafficking and surface expression of the TCR complex.

      • Properties
        • Host
          syrian hamster
          Isotype
          IgG2a kappa
          Reactivity
          mouse
          Clone
          500A2
          Immunogen
          Mouse T-cell receptor
          Purification
          >95% by SDS-PAGE and HPLC
          Formulation
          0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
          Storage
          anti-CD3e In Vivo Antibody - Low Endotoxin (500A2) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
          Molecular Weight
          1.0 mg/ml
          Regulatory Status
          Functional Assay: Binding of Hamster Anti-Mouse CD3ε (Clone 500 A2) to CD3 initiates the intracellular biochemical pathway resulting in cellular activation and proliferation.
          Each investigator should determine their own optimal working dilution for specific applications.

          * Products are for research use only. Not for use in diagnostic or therapeutic procedures.

      • Applications
        • Application
          Functional Assays; Flow Cytometry; IHC (frozen)

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