Name: Anti-Mouse CD4 In Vivo Antibody - Low Endotoxin (GK1.5)
Price: 680.00 USD

- Overview
  - Antigen Distribution: The L3T4 antigen is expressed by the helper/inducer subset of mouse T-cells. The antigen is present on approximately 80% of thymocytes, 20% of spleen cells and 60% of lymph node cells. The expression of L3T4 correlates with class II MHC antigen reactivity on cloned T-cell lines.
    Specificity: Anti-CD4 In Vivo Antibody - Low Endotoxin (GK1.5) recognizes an epitope on Mouse CD4
    Purification method: This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
    Isotype Control: Rat IgG2b In Vivo Isotype Control - Low Endotoxin [1-2] (ICH2243)
    Endotoxin: ≤ 1.0 EU/mg as determined by the LAL method
    Aggregation: Aggregation level ≤ 5%
    
    Please contact us at for specific academic pricing.
    
    Background
    
    CD4 (cluster of differentiation 4) is a glycoprotein expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells. CD4 interacts with class II molecules of the major histocompatibility complex (MHC) enhancing the signal for T-cell activation.
- Properties
  - Host
    
    rat
    
    Isotype
    
    IgG2b kappa
    
    Reactivity
    
    mouse
    
    Clone
    
    GK1.5
    
    Immunogen
    
    Mouse CTL clone V4
    
    Purification
    
    >95% by SDS-PAGE and HPLC
    
    Formulation
    
    0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
    
    Storage
    
    anti-CD4 In Vivo Antibody - Low Endotoxin (GK1.5) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
    
    Molecular Weight
    
    ≥ 2.0 mg/ml
    
    Regulatory Status
    
    Blocking: Anti-Mouse CD4 (clone GK1.5) is useful for In vitro and In vivo studies of T-helper cell functions including blocking of ligand binding and T-helper cell activation.
    Each investigator should determine their own optimal working dilution for specific applications.
    
    Antigen
    
    (Gene ID:T-cell differentiation antigen L3T4, T-cell surface antigen T4/Leu-3)
    
    * Products are for research use only. Not for use in diagnostic or therapeutic procedures.
- Applications
  - Application
    
    WB; Blocking; Flow Cytometry; IHC (frozen); Immunoprecipitation

Anti-Mouse CD4 In Vivo Antibody - Low Endotoxin (GK1.5)

Anti-Mouse CD4 In Vivo Antibody - Low Endotoxin (GK1.5)

Background

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