Name: Anti-Mouse CD3e In Vivo Antibody - Low Endotoxin (145-2C11)
Price: 680.00 USD

- Overview
  - Antigen Distribution: The CD3 complex is present on thymocytes (differentiation dependent), mature T cells, NK-T cells of all mouse strains tested.
    Specificity: Anti-CD3e In Vivo Antibody - Low Endotoxin (145-2C11) recognizes an epitope on Mouse CD3e
    Purification method: This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
    Isotype Control: Armenian Hamster IgG Isotype Control for In Vivo - Low Endotoxin [PIP] (ICH2251)
    Endotoxin: ≤ 1.0 EU/mg as determined by the LAL method
    Aggregation: Aggregation level ≤ 5%
    
    Please contact us at for specific academic pricing.
    
    Background
    
    CD3e is a 20 kD transmembrane protein and is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.
- Properties
  - Host
    
    armenian hamster
    
    Isotype
    
    IgG
    
    Reactivity
    
    mouse
    
    Clone
    
    145-2C11
    
    Immunogen
    
    H-2Kb-specific mouse cytotoxic T lymphocyte clone BM10-37
    
    Purification
    
    >95% by SDS-PAGE and HPLC
    
    Formulation
    
    0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
    
    Storage
    
    anti-CD3e In Vivo Antibody - Low Endotoxin (145-2C11) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
    
    Molecular Weight
    
    1.0 mg/ml
    
    Regulatory Status
    
    Blocking: The 145-2C11 antibody has been reported to block the binding of 17A2 antibody to CD3 epsilon-specific T cells
    Each investigator should determine their own optimal working dilution for specific applications.
    
    Antigen
    
    (Gene ID:T3/Leu-4 epsilon chain, CD3 epsilon chain, CD3e)
    
    * Products are for research use only. Not for use in diagnostic or therapeutic procedures.
- Applications
  - Application
    
    IHC (frozen); Flow Cytometry; Immunoprecipitation; WB; In vivo Depletion; Blocking

Anti-Mouse CD3e In Vivo Antibody - Low Endotoxin (145-2C11)

Anti-Mouse CD3e In Vivo Antibody - Low Endotoxin (145-2C11)

Background

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