Anti-Ki-67 Antibody-W

Anti-Ki-67 Antibody-W

Catalog Number:
A001435919FLU
Mfr. No.:
Ki-67-Antibody-W
Price:
$450
  • Size:
    Vial
    Quantity:
    Add to Cart:
      • Overview
        • Ki-67 Antibody-W reacts against the Ki-67 protein. The Ki-67 protein is an intrinsic marker of ongoing cell proliferation and is present during all active phases of the cell cycle (G1, S, G2 and mitosis) and is absent from resting cells (G0). This means it is an excellent marker for determining the growth fraction of a given cell population. It is used extensively in diagnostic, research and drug discovery applications. Ki-67 Antibody-W is polyclonal and was raised in rabbit to the human peptide sequence -TPKEKAQALEDLAGFKELFQT - that was coupled to thyroglobulin by glutaraldehyde before injection into the rabbit. Our antibody has been used for immunocytochemistry in rat and human brain tissue. It will be sold with filter paper absorbed with the specific peptide for blocking. This allows the researcher to confirm the specific signal

          Please contact us at for specific academic pricing.

          Background

          The Ki-67 Antibody-W, GFAP Antibody-W, and BrdU Antibody-W are excellent antibodies that are recent introductions. Included with these antibodies is filter paper absorbed with a specific blocking peptide. We feel that a blocking agent should be utilized in all investigations using these types of antibodies. Only with a blocking agent can the researcher verify and have confidence that the observed signal is the intended signal. At no additional costs, we have included the blocking agent, which makes this verification easy and accurate for the researcher to implement.

      • Properties
        • Storage
          The vial you will receive contains approximately 200µl of solution at a dilution of 1/100. The Ki-67Antibody-W can be further diluted to at least 1/20,000 in BSA diluent (50mM KPBS, 0.4% Triton, 1% BSA, 1% NGS).
          The antibody should be stored at -20C. Also included is filter paper absorbed with the specific peptide for blocking. 500µl of the diluted antibody can be added to the tube to achieve a 10µM block. Incubate for at least 1 hour at room temperature (RT) before applying to tissue. The antibody blocks specifically with this peptide and does not block with other peptides absorbed on filter paper

          * For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

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