Anti-BrdU Antibody-W

This antibody reacts against the synthetic thymidine analog BrdU, which when injected into an animal is incorporated during the S phase of the cell cycle into newly synthesized DNA strands of actively proliferating cells. The number of cells that survive can be measured by immunocytochemistry. As an outstanding tool to assess the proliferation state of a group of cells, it has become vital to drug discovery research, especially with cancer therapeutics and ascertaining the health of cells during ADME/Tox studies. BrdU Antibody-W is a polyclonal raised in rabbit against BrdU conjugated to bovine serum albumin (BSA). Our antibody has been used for immunocytochemistry in rat brain tissue. It will be sold with filter paper absorbed with BrdU for blocking. This allows the researcher to confirm the specific signal.

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Background

The Ki-67 Antibody-W, GFAP Antibody-W, and BrdU Antibody-W are excellent antibodies that are recent introductions. Included with these antibodies is filter paper absorbed with a specific blocking peptide. We feel that a blocking agent should be utilized in all investigations using these types of antibodies. Only with a blocking agent can the researcher verify and have confidence that the observed signal is the intended signal. At no additional costs, we have included the blocking agent, which makes this verification easy and accurate for the researcher to implement.