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Overview
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Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21. 3.
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Overview
Immunoprecipitation analysis of 150ug extracts of MCF7 cells using 3ug Histone H3 antibody (STJ116178). Western blot was performed from the immunoprecipitate using Histone H3 antibody (STJ116178) at a dilition of 1:500.
Immunohistochemistry of paraffin-embedded mouse kidney using Histone H3 antibody (STJ116178) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using Histone H3 antibody (STJ116178) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using Histone H3 antibody (STJ116178) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using Histone H3 antibody (STJ116178) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.
Anti-HIST3H3 antibody
Anti-HIST3H3 antibody
Catalog Number:
A001023047STJ
Mfr. No.:
STJ116178
Price:
$297
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