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Overview
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Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene has sequence homology to members of the histone deacetylase family. This gene is orthologous to mouse HDAC7 gene whose protein promotes repression mediated via the transcriptional corepressor SMRT. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
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Overview
Western blot analysis of extracts of various cell lines, using HDAC7 antibody (STJ29424) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Enhanced Kit.Exposure time: 30s.
Immunofluorescence analysis of MCF-7 cells using HDAC7 antibody (STJ29424). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using HDAC7 antibody (STJ29424) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.
Anti-HDAC7 antibody
Anti-HDAC7 antibody
Catalog Number:
A001037408STJ
Mfr. No.:
STJ29424
Price:
$297
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