Name: Anti-GPR94/TRA1 antibody
Price: 499.00 USD

- Overview
  - Please contact us at for specific academic pricing.
- Properties
  - Host
    
    goat
    
    Antibody Type
    
    polyclonal
    
    Reactivity
    
    human; mouse; rat; dog; pig; cow; zebrafish
    
    Purification
    
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    
    Conjugate
    
    Unconjugated
    
    Formulation
    
    Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
    
    Storage
    
    Store at -20°C and avoid freeze-thaw cycles.
    
    Concentration
    
    0.5 mg/ml
    
    Regulatory Status
    
    RUO
    
    Antigen
    
    Endoplasmin; 94 Kda Glucose-Regulated Protein; Grp-94; Heat Shock Protein 90 Kda Beta Member 1; Tumor Rejection Antigen 1; Gp96 Homolog (Gene ID:7184; UniProt:P14625)
    
    * For research use only
- Applications
  - Application
    
    ELISA; WB; IHC; IF; Flow Cytometry
    
    Application Description
    
    ELISA-antibody detection limit dilution 1:32000.
    WB-Approx 100kDa band observed in lysates of cell lines HEK293, A549, HeLa, U2OS and NIH3T3 and in preliminary testing of MCF7 and HepG2 cell lysate. This molecular weight is routinely observed by other sources. Recommended concentration: 0.1-0.3µg/ml. Primary incubation 1 hour at room temperature.
    IHC-Paraffin embedded Human Uterus and Liver. Recommended concentration: 3-5µg/ml.
    IF-Strong expression of the protein seen in the ER and cytoplasm of A431 and HeLa cells. Recommended concentration: 10µg/ml.
    FC-Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.