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STJ70949 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (b STJ70949 (0.03µg/ml) staining of HepG2 (A) and NIH3T3 (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-HSPA8 antibody

Anti-HSPA8 antibody

Catalog Number:

A001042749STJ

Mfr. No.:

STJ70949

Price:

$499

Size:

100 µg

Quantity:

Add to Cart:

- Overview
  - Please contact us at for specific academic pricing.
- Properties
  - Host
    
    goat
    
    Antibody Type
    
    polyclonal
    
    Reactivity
    
    human; mouse; rat; dog
    
    Purification
    
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    
    Conjugate
    
    Unconjugated
    
    Formulation
    
    Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
    
    Storage
    
    Store at -20°C and avoid freeze-thaw cycles.
    
    Concentration
    
    0.5 mg/ml
    
    Regulatory Status
    
    RUO
    
    Antigen
    
    Heat Shock Cognate 71 Kda Protein; Heat Shock 70 Kda Protein 8; Lipopolysaccharide-Associated Protein 1; Lap-1; Lps-Associated Protein 1 (Gene ID:3312; UniProt:P11142)
    
    * For research use only
- Applications
  - Application
    
    ELISA; IF; WB
    
    Application Description
    
    ELISA-antibody detection limit dilution 1:4000.
    WB-Approx 75kDa band observed in lysates of cell lines HepG2 and NIH3T3. This molecular weight is routinely observed by other sources. Recommended concentration: 0.03-0.1µg/ml. Primary incubation 1 hour at room temperature.
    IF-Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10µg/ml

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