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Overview
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This gene encodes the largest subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. Dynactin is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit interacts with dynein intermediate chain by its domains directly binding to dynein and binds to microtubules via a highly conserved glycine-rich cytoskeleton-associated protein (CAP-Gly) domain in its N-terminus. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. Mutations in this gene cause distal hereditary motor neuronopathy type VIIB (HMN7B) which is also known as distal spinal and bulbar muscular atrophy (dSBMA).
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- Properties
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Overview
Immunofluorescence analysis of HeLa cells using DCTN1 antibody (STJ23347). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of MCF-7 cells, using 3 ug DCTN1 antibody (STJ23347). Western blot was performed from the immunoprecipitate using DCTN1 antibody (STJ23347) at a dilition of 1:1000.
Western blot analysis of extracts of various cell lines, using DCTN1 antibody (STJ23347) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.
Anti-DCTN1 antibody
Anti-DCTN1 antibody
Catalog Number:
A001033737STJ
Mfr. No.:
STJ23347
Price:
$297
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