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Overview
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Cyclophilin B is a protein encoded by the PPIB gene which is approximately 23, 7 kDa. Cyclophilin B is localised to the endoplasmic reticulum lumen and melanosome. It is involved in collagen chain trimerization, degradation of the extracellular matrix and the prolactin signalling pathway. It is a cyclosporine-binding protein that binds to cells derived from T-and B-lymphocytes, and may regulate cyclosporine A-mediated immunosuppression. Cyclophilin B is expressed in the skin, nervous system, liver, lung and stomach. Mutations in the PPIB gene may result in osteogenesis imperfecta. STJ97530 was developed from clone 2B10 and was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. This primary antibody detects endogenous levels of Cyclophilin B.
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Overview
Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1, Cyclophilin B monoclonal antibody (2B10) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Western blot analysis of 1) Jurkat, 2) 293T, 3) Rat Liver Tissue, 4) 3T3 with Cyclophilin B Mouse mAb diluted at 1:2, 000.
Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1, Cyclophilin B monoclonal antibody (2B10) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1, Cyclophilin B monoclonal antibody (2B10) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunofluorescence analysis of rat-spleen tissue. 1, Cyclophilin B monoclonal antibody (2B10) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B Immunofluorescence analysis of human-lung tissue. 1, Cyclophilin B monoclonal antibody (2B10) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B Immunofluorescence analysis of Hela cell. 1, Calmodulin Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). (red) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).
Anti-Cyclophilin B antibody
Anti-Cyclophilin B antibody
Catalog Number:
A001052898STJ
Mfr. No.:
STJ97530
Price:
$224
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