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Overview
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This gene is a member of the cullin family. The encoded protein forms a complex that functions as an E3 ubiquitin ligase and catalyzes the polyubiquitination of specific protein substrates in the cell. The protein interacts with a ring finger protein, and is required for the proteolysis of several regulators of DNA replication including chromatin licensing and DNA replication factor 1 and cyclin E. Multiple transcript variants encoding different isoforms have been found for this gene.
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Overview
Immunofluorescence analysis of U2OS cells using CUL4B antibody (STJ27353).
Immunofluorescence analysis of U2OS cells using CUL4B antibody (STJ27353). Blue: DAPI for nuclear staining. DNA damage by a UV-A laser.
Immunohistochemistry of paraffin-embedded human liver cancer using CUL4B antibody (STJ27353) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse pancreas using CUL4B antibody (STJ27353) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat liver using CUL4B antibody (STJ27353) at dilution of 1:200 (40x lens).
Western blot analysis of extracts of various cell lines, using CUL4B antibody (STJ27353) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.
Anti-CUL4B antibody
Anti-CUL4B antibody
Catalog Number:
A001035821STJ
Mfr. No.:
STJ27353
Price:
$297
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