Name: Anti-Collagen IV antibody
Price: 224.00 USD

Overview
- Collagen IV is a protein encoded by the COL4A1 gene which is approximately 160, 6 kDa. Collagen IV is secreted into the extracellular space. It is involved in collagen chain trimerization, the integrin pathway, ERK signalling and focal adhesion. It is the major structural component of glomerular basement membranes and forms a chicken-wire meshwork together with laminins, proteoglycans and entactin/nidogen. It specifically inhibits endothelial cell proliferation, migration and tube formation. Collagen IV is expressed in the nervous system, liver, lung, heart and kidney. Mutations in the COL4A1 gene may result in the tortuosity of retinal arteries. STJ98907 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This antibody detects endogenous Collagen IV protein.
  
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Properties
- Host
  
  mouse
  
  Antibody Type
  
  monoclonal
  
  Reactivity
  
  human; mouse; rat
  
  Immunogen
  
  synthetic peptide derived from Collagen IV.
  
  Purification
  
  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  
  Conjugate
  
  Unconjugated
  
  Formulation
  
  Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  
  Storage
  
  Store at -20°C and avoid repeat freeze-thaw cycles.
  
  Concentration
  
  1 mg/ml
  
  Regulatory Status
  
  RUO
  
  Antigen
  
  Collagen Alpha-1(Iv Chain [Cleaved Into-Arresten] (Gene ID:1282; UniProt:p02462)
  
  * For research use only

Applications

Application

IF; IHC (paraffin)

Application	Dilutions
IF	1:50-200
IHC (paraffin)	1:50-300

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Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Immunohistochemical analysis of paraffin-embedded Human colon. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Immunohistochemical analysis of paraffin-embedded Human colon. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Immunohistochemical analysis of paraffin-embedded Human colon. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Anti-Collagen IV antibody

Anti-Collagen IV antibody