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Overview
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Collagen I is a protein encoded by the COL1A1 gene which is approximately 138, 9 kDa. Collagen I is secreted into the extracellular space. It is involved in collagen chain trimerization, the integrin pathway, ERK signalling, focal adhesion and the phospholipase-C pathway. It is a pro-alpha-1 chain of type I collagen whose triple helix comprises two alpha-1 chains and one alpha-2 chain. Collagen I is expressed in the bone, skin, eye, lung and nervous system. Mutations in the COL1A1 gene may result in Caffey disease and Ehlers-Danlos syndrome. STJ98915 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This antibody detects endogenous Collagen I protein.
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Overview
Immunohistochemical analysis of paraffin-embedded Human skin. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
Immunohistochemical analysis of paraffin-embedded Human skin. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1, Collagen I Mouse monoclonal antibody (4H10) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human skin. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
Immunohistochemical analysis of paraffin-embedded Human-lung-cancer tissue. 1, Collagen I Mouse monoclonal antibody (4H10) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Anti-Collagen I antibody
Anti-Collagen I antibody
Catalog Number:
A001054282STJ
Mfr. No.:
STJ98915
Price:
$224
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