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Overview
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This gene encodes a TAF (TATA box-binding protein-associated factor), which associates with TBP (TATA box-binding protein) to form the B-TFIID complex that is required for transcription initiation of genes by RNA polymerase II. This TAF has DNA-dependent ATPase activity, which drives the dissociation of TBP from DNA, freeing the TBP to associate with other TATA boxes or TATA-less promoters.
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- Properties
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Overview
Immunoprecipitation analysis of 150ug extracts of HeLa cells using 3ug BTAF1 antibody (STJ28374). Western blot was performed from the immunoprecipitate using BTAF1 antibody (STJ28374) at a dilition of 1:500.
Chromatin immunoprecipitation analysis of extracts of HeLa cells, using BTAF1 antibody (STJ28374) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Western blot analysis of extracts of various cell lines, using BTAF1 antibody (STJ28374) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.
Anti-BTAF1 antibody
Anti-BTAF1 antibody
Catalog Number:
A001036476STJ
Mfr. No.:
STJ28374
Price:
$297
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