Name: Anti-NSE antibody
Price: 224.00 USD

Overview
- NSE is a protein encoded by the ENO2 gene which is approximately 47, 2 kDa. NSE is localised to the cytoplasm and cell membrane. It is involved in glucose metabolism, carbon metabolism, the HIF-1 signalling pathway and deadenylation-dependent mRNA decay. It is one of the three enolase isoenzymes found in mammals. It has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system neurons and binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival. NSE is expressed in the cells of the nervous system, eye, blood, liver and kidney. Mutations in the ENO2 gene may result in a granular cell tumour. STJ96968 was developed from clone 13E2 and was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. This primary antibody detects endogenous NSE proteins.
  
  Please contact us at for specific academic pricing.
Properties
- Host
  
  mouse
  
  Antibody Type
  
  monoclonal
  
  Isotype
  
  IgG1
  
  Reactivity
  
  human; mouse; rat
  
  Clone
  
  13E2
  
  Immunogen
  
  Synthetic Peptide
  
  Purification
  
  The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
  
  Conjugate
  
  Unconjugated
  
  Formulation
  
  PBS, pH 7.4, containing 0.5% BSA, 0.02% sodium azide and 50% Glycerol.
  
  Storage
  
  Store at -20°C and avoid repeat freeze-thaw cycles.
  
  Regulatory Status
  
  RUO
  
  Antigen
  
  Gamma-Enolase; 2-Phospho-D-Glycerate Hydro-Lyase; Enolase 2; Neural Enolase; Neuron-Specific Enolase; Nse (Gene ID:2026; UniProt:P09104)
  
  * For research use only

Applications

Application

WB; IF; IHC (paraffin)

Application	Dilutions
WB	1:2000
IHC	1:200
IF	1:200

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Immunohistochemical analysis of paraffin-embedded Human pancreas. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Immunohistochemical analysis of paraffin-embedded Human pancreas. 1, Antibody was diluted at 1:400 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Immunohistochemical analysis of paraffin-embedded Human pancreas. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Immunohistochemical analysis of paraffin-embedded Human pancreas. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Immunohistochemical analysis of paraffin-embedded Human pancreas. 1, Antibody was diluted at 1:100 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Immunohistochemical analysis of paraffin-embedded Human pancreas. 1, Antibody was diluted at 1:100 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

IHC staining of Human small cell carcinoma of lung tissue, diluted at 1:200.

Immunohistochemical analysis of paraffin-embedded Human pancreas. 1, Antibody was diluted at 1:400 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Immunohistochemical analysis of paraffin-embedded Human pancreas. 1, Antibody was diluted at 1:100 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Immunohistochemical analysis of paraffin-embedded Human pancreas. 1, Antibody was diluted at 1:400 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Anti-NSE antibody

Anti-NSE antibody