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Overview
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This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone.
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- Properties
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Overview
Immunofluorescence analysis of NIH-3T3 cells using ACAT1 antibody (STJ115238) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using ACAT1 antibody (STJ115238) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of C6 cells using ACAT1 antibody (STJ115238) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunohistochemistry of paraffin-embedded mouse kidney using ACAT1 antibody (STJ115238) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat ovary using ACAT1 antibody (STJ115238) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using ACAT1 antibody (STJ115238) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using ACAT1 antibody (STJ115238) at 1:5000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.
Anti-ACAT1 antibody
Anti-ACAT1 antibody
Catalog Number:
A001022137STJ
Mfr. No.:
STJ115238
Price:
$297
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