anti-AAV8 (intact particle) mouse monoclonal, ADK8, supernatant

Overview
- For characterization of different stages of infection and very useful for analysis of the AAV assembly process. ADK8 specifically reacts with AAV1, AAV3, AAV7, AAV8, AAVrh10, AAVrh74 and Anc80, empty and full capsids. Recognizes a conformational epitope of assembled capsids. Not for use in immunoblotting.
  The ADK8 antibody is used as capture and detection antibody in the AAV8 Titration ELISA (PRAAV8), the AAVrh10 Titration ELISA (PRAAV10) and the AAV8 Xpress ELISA (PRAAV8XP).
  
  Please contact us at for specific academic pricing.
  
  Background
  
  For characterization of different stages of infection and very useful for the analysis of the AAV assembly process. ADK8 specifically reacts with AAV1, AAV3, AAV7, AAV8, AAVrh10, AAVrh74 and Anc80, empty and full capsids. Recognizes a conformational epitope of assembled capsids. Predicted binding site: residues 586-LQQQNT-591 (Gurda et al., 2012). The antibody cannot be used for immunoblotting using denaturing conditions.
  The antibody is also useful for neutralizing experiments.
  
  Gurda BL, et al. Mapping a neutralizing epitope onto the capsid of adeno-associated virus serotype 8. J Virology 86, 7739-7751 (2012).
  
  More Details
  
  AAV Antibodies
Properties
- Host
  
  mouse
  
  Antibody Type
  
  monoclonal
  
  Isotype
  
  IgG2a kappa
  
  Reactivity
  
  virus (AAV1, AAV3, AAV7, AAV8, AAVrh10, AAVrh74, Anc80)
  
  Clone
  
  ADK8
  
  Immunogen
  
  AAV8 capsids
  
  Purification
  
  hybridoma cell culture supernatant
  
  Conjugate
  
  Unconjugated
  
  Formulation
  
  contains 0.09% sodium azide
  
  Storage
  
  short term at 2-8°C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
  
  * Research use only

Applications

Application

Dot Blots; ELISA; ICC/IF; IP; Neutralization Assay

Application	Dilutions
Dot Blot	assay dependent (non-denaturing conditions)
ELISA	assay dependent
Immunocytochemistry (ICC)/ Immunofluorescence (IF)	assay dependent
Immunoprecipitation (IP)	assay dependent
Neutralization Assay	assay dependent

Application	Species	Publication
Dot Blots	AAV3 AAV8	PMID: 27424005
ELISA	AAV1 AAV3 AAV7 AAV8 AAVrh10	PMID: 24299301
ICC/IF	AAV8	PMID: 22593150
IP	AAV8	PMID: 22718833
Neutralization Assay	AAV3 AAV8 AAV9	PMID: 21917944

Reference
- Tseng, Y.-S. et al. Generation and characterization of anti-Adeno-associated virus serotype 8 (AAV8) and anti-AAV9 monoclonal antibodies. J. Virol. Methods 236, 105-110 (2016).
  Mietzsch, M. et al. OneBac: Platform for Scalable and High-Titer Production of Adeno-Associated Virus Serotype 1-12 Vectors for Gene Therapy. Hum. Gene Ther. 25, 212-222 (2014).
  Gurda, B. L. et al. Mapping a Neutralizing Epitope onto the Capsid of Adeno-Associated Virus Serotype 8. J. Virol. 86, 7739-7751 (2012).
  Raupp, C. et al. The Threefold Protrusions of Adeno-Associated Virus Type 8 Are Involved in Cell Surface Targeting as Well as Postattachment Processing. J. Virol. 86, 9396-9408 (2012).
  Sonntag, F. et al. The Assembly-Activating Protein Promotes Capsid Assembly of Different Adeno-Associated Virus Serotypes. J. Virol. 85, 12686-12697 (2011).