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Overview
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The AAV8 ELISA Control consists of fully assembled, empty AAV8 capsids. It represents the serotype-specific conformational epitopes and is therefore a reliable positive control in detection and quantification assays based on AAV antibodies detecting the conformational epitopes, such as ELISA.
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Background
The AAV8 ELISA Control consists of fully assembled, empty AAV8 capsids. The concentration is lot specific.
Ayuso, E. et al. Manufacturing and characterization of a recombinant adeno-associated virus type 8 reference standard material. Hum. Gene Ther. 25, 977–987 (2014).
The AAV8 ELISA Control can be used as a positive control with PROGEN’s AAV8 Titration ELISA (PRAAV8). If you require different lots of the AAV8 ELISA Control and the Kit Control included in your PROGEN AAV8 Titration ELISA, please enquire to check availability.
Reading of the AAV8 ELISA Control from the standard curve (i.e. Kit Control, included in PROGEN’s AAV8 Titration ELISA) is influenced by inter- and intra-assay, but also by inter-lab variances. Therefore, it is recommended that each laboratory determines its own acceptable range of recovery.
The AAV8 ELISA Control has been calibrated on the ATCC AAV8 reference standard material (RSM, VR-1816). For further information, please see the publication by Ayuso et al., 2014.
D’Costa, S. et al. Practical utilization of recombinant AAV vector reference standards: focus on vector genomes titration by free ITR qPCR. Mol. Ther. Methods Clin. Dev. 5, 16019 (2016).
Gurda, B. L. et al. Mapping a neutralizing epitope onto the capsid of adeno-associated virus serotype 8. J. Virol. 86, 7739–7751 (2012).
Sonntag, F. et al. The Assembly-Activating Protein Promotes Capsid Assembly of Different Adeno-Associated Virus Serotypes. J. Virol. 85, 12686–12697 (2011).
More Details
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- Properties
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Overview
Alternative Products
| Catalog Number | Product Name | Format | Price |
|---|---|---|---|
| PRAAV1-C | AAV1 ELISA Control | 1 vial (0.6E+09 – 1.0E+09 capsids) | $312 |
| PRAAV2-C | AAV2 ELISA Control | 1 vial (0.8E+09 – 1.4E+09 capsids) | $312 |
| PRAAV3-C | AAV3 ELISA Control | 1 vial (5.5E+08 – 9.5E+08 capsids) | $312 |
| PRAAV5-C | AAV5 ELISA Control | 1 vial (3.9E+09 – 6.5E+09 capsids) | $312 |
| PRAAV6-C | AAV6 ELISA Control | 1 vial (1.0E+09 – 1.6E+09 capsids) | $312 |
| PRAAV9-C | AAV9 ELISA Control | 1 vial (4.5E+08 – 7.5E+08 capsids) | $312 |
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